Pathology Handbook

11 - Deoxycortisol

Clinical Indications

11 deoxycortisol is used in the diagnosis and monitoring of congenital adrenal hypoplasia due to 11ß-hydroxylase deficiency. The assay should be requested when CAH is suspected on clinical grounds but serum 17-OH progesterone levels are equivocal. It is analysed by Tandem mass spectrometry and a cortisol result is also reported.

Request Form 

ICE request

Availability

Run weekly in referral laboratory.

Specific Criteria

Collect at 09:00hrs as there is diurnal variation.

Patient Preparation

The patient must not be on steroids.

Turnaround Time

7 working days.

Volume

200 µL minimum volume is required.

Container

Red topped Vacutainer serum or Lithium heparin plasma.

Vacutainer red top

Lab Handling

Separated and sent to a referral laboratory for analysis (Wythenshaw Hospital, Manchester)

Causes for Rejection

Unlabelled or incorrectly labelled sample.

Taken at inappropriate time.

Request not justified.

Reference Range

See referral lab report form.