Pathology Handbook

Adrenal Cortex Antibody

Clinical Indications

Addison's disease.

Please note: Autoantibodies to adrenal cortex are found in approximate 50% of patients with Addisonian adrenal insufficiency where there are other autoimmune diseases, although the prevalence drops when the autoimmune adrenalitis occurs alone. Autoimmune adrenal disease is closely associated with other organ-specific autoimmune disease, including thyrogastric (Schmidt's syndrome) and parathyroid autoimmune disease, and it is therefore important to screen for thyroid and gastric parietal cell antibodies.

Test Includes

Detection of IgG autoantibodies against the adrenal cortex.

Request Form 

Request via ICE


Assay is performed twice weekly.

Specific Criteria

It is essential to provide relevant clinical information.

Patient Preparation

No special preparation is required.

Turnaround Time

All samples are generally sent to  referral laboratory  within 3 working days of receipt in the laboratory. Samples are batched and the assay is performed twice per week.


Serum in red top tube

Gold top (SST) tube will be accepted


7 ml


Vacutainer red top


Samples should be transported to the laboratory as soon as is practicably possible.

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample.

Inadequately completed request form.

Delay in sample reaching laboratory.

Reference Range

Samples are tested for the presence of antibodies to adrenal cortex by indirect immunofluorescence and the result reported as Positive or Negative.