Associated tumours: breast cancer; small
cell lung carcinoma.
(Specialist request only) Neurological
symptoms: Stiff person's syndrome;
encephalomyelitis; subacute sensory neuropathy; sensorimotor
Detection of IgG autoantibodies to amphiphysin in cerebellar
presynaptic nerve terminals.
Request via ICE
Assay is performed weekly.
It is essential to provide relevant clinical information.
No special preparation is required.
All samples are generally sent to referral
laboratory within 3 working days of receipt in the
laboratory. Samples are batched and the assay is performed twice
Serum in red top tube
Gold top (SST) tube will be accepted
Samples should be transported to the laboratory as soon as is
Aliquot and store at 4-8°C prior to testing and at -20°C or
below for up to 1 month after receipt.
Causes for Rejection
Unlabelled/inadequately labelled sample.
Inadequately completed request form.
Delay in sample reaching laboratory.
Samples are tested for the presence of antibodies to amphiphysin
by indirect immunofluorescence and the result reported as a titre.
Confirmation is by western blot analysis.