Pathology Handbook

Amphiphysin Antibody

Clinical Indications

Associated tumours: breast cancer; small cell lung carcinoma.

(Specialist request only) Neurological symptoms:  Stiff person's syndrome; encephalomyelitis; subacute sensory neuropathy; sensorimotor peripheral neuropathy

Test Includes

Detection of IgG autoantibodies to amphiphysin in cerebellar presynaptic nerve terminals.

Request Form 

Request via ICE


Assay is performed weekly.

Specific Criteria

It is essential to provide relevant clinical information.

Patient Preparation

No special preparation is required.

Turnaround Time

All samples are generally sent to referral laboratory within 3 working days of receipt in the laboratory. Samples are batched and the assay is performed twice per week.


Serum in red top tube

Gold top (SST) tube will be accepted


7 ml


Vacutainer red top


Samples should be transported to the laboratory as soon as is practicably possible.

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample.

Inadequately completed request form.

Delay in sample reaching laboratory.

Reference Range

Samples are tested for the presence of antibodies to amphiphysin by indirect immunofluorescence and the result reported as a titre. Confirmation is by western blot analysis.