Anti Neutrophil Cytoplasmic Antibody
ANCA, ANCA IIF
Please note: A positive result should not be
considered diagnostic as antibodies may be found in other
conditions including inflammatory bowel disease or ulcerative
colitis. All other factors such as clinical history and all other
serology and/or biopsy results must be taken into account. Also,
not all patients with small vessel vasculitis will be positive for
ANCA. All factors including clinical history and any radiological
and histological results must also be considered
Detection of IgG autoantibodies against a variety of human
Request via ICE
Assay performed daily Monday to Friday
It is essential to inform the laboratory by telephone of any
It is essential to provide relevant clinical information
All tests are performed on the next routine working day after
receipt of the sample.
Samples should be transported to the laboratory immediately
Aliquot and store at 4-8°C prior to testing and at -20°C
or below for up to 1 month after receipt.
Causes for Rejection
Unlabelled/inadequately labelled sample.
Inadequately completed request form.
Delay in sample reaching laboratory.
Sera are screened at a dilution of 1/20. Any sample found to be
ANCA or ANA positive at this dilution will be tested for the
presence of MPO and PR3 ANCA