Pathology Handbook

Anti Neutrophil Cytoplasmic Antibody



Clinical Indications

Wegener's granulomatosis
Microscopic polyangiitis
Crescentic glomerulonephritis
Churg-Strauss syndrome

Please note: A positive result should not be considered diagnostic as antibodies may be found in other conditions including inflammatory bowel disease or ulcerative colitis. All other factors such as clinical history and all other serology and/or biopsy results must be taken into account. Also, not all patients with small vessel vasculitis will be positive for ANCA. All factors including clinical history and any radiological and histological results must also be considered

Test Includes

Detection of IgG autoantibodies against a variety of human neutrophil antigens

Request Form 

Request via ICE


Assay performed daily Monday to Friday

Specific Criteria

It is essential to inform the laboratory by telephone of any urgent requests.
It is essential to provide relevant clinical information

Turnaround Time

All tests are performed on the next routine working day after receipt of the sample.




7 ml


Vacutainer red top


Samples should be transported to the laboratory immediately

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample.
Inadequately completed request form.
Delay in sample reaching laboratory.

Reference Range

Sera are screened at a dilution of 1/20. Any sample found to be ANCA or ANA positive at this dilution will be tested for the presence of MPO and PR3 ANCA