Antenatal Sickle and Thalassaemia screening
FOQ, AN Thalassaemia screen, Sickle screen, Haemoglobin variant,
Testing as part of national screening programme of all antenatal
patients for the early detection of haemoglobin abnormalities
including sickle cell and thalassaemia conditions.
FBC and HPLC (if indicated by the information provided on Family
Origin Questionnaire (FOQ) and/ or from the FBC indices).
Additional test/ s may be performed if the HPLC results detect a
possible abnormality these include:-Sickle solubility test,
cellulose acetate electrophoresis and Kleihauer film
Request via ICE plus FOQ
On request; daily routine working day.
Ethnic origin required; Test must be performed within 10 weeks
of pregnancy and a FOQ sent with sample.
Genetic counselling must be considered. Patient consent required
for completion of FOQ.
An Interim report will always be issued within 3 days where full
confirmatory tests are not yet completed.
Purple top (EDTA) tube
Causes for Rejection
Incorrect sample identification, clotted, inadequate, grossly
haemolysed samples. Previously reported (x2) Haemoglobinopathy
The national programme algorithm is used for reporting results
and suggesting follow up testing, which may include partner testing
/ DNA analysis. Positive results are sent to the Antenatal
Screening co-ordinator at Southend Hospital who will contact the
patient directly to arrange further testing / counselling/
Hb variants requiring confirmation / DNA/ globin chain analysis
will be sent to Reference Centre