Pathology Handbook

Anti ds DNA

Synonyms

Anti DNA
DNA binding
Double stranded DNA

Clinical Indications

SLE
'Lupoid' chronic active hepatitis
Please note: Elevated levels occur predominantly in SLE. They are not found in other connective tissue disorders, or in all cases of SLE

Test Includes

Detection of IgG autoantibodies against double stranded DNA

Request Form 

Request via ICE

Availability

Assay performed daily; Monday to Friday.

Specific Criteria

It is essential to provide relevant clinical information.
Only samples found to be ANA positive will be referred for ds DNA testing unless clinical information suggests testing may be relevant

Turnaround Time

The assay is performed daily; Monday to Friday.

Specimen

Serum

Volume

7 ml

Container

Vacutainer red top

Collection

Samples should be transported to the laboratory immediately

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample
Inadequately completed request form
Delay in sample reaching laboratory

Grossly haemolysed, icteric or lipaemic samples may interfere with the assay and will be rejected.

Reference Range

EIA:

Negative: <10 IU/mL

Equivocal: 10-15 IU/mL

Positive: >15 IU/mL

IIF:

Samples are screened at a dilution of 1:10