Pathology Handbook

Chlamydia Detection

Test Includes

Detection of Chlamydia trachomatis by RNA polymerase chain reaction.

Clinical Indications

Suspected Chlamydia trachomatis infections of the genito-urinary tract or eye.

Request Form

ICE request or National Chlamydia Screening Programme form where appropriate.

Availability

On request during working hours Monday to Friday

Specific Criteria

Inadequately collected samples may result in false negative results.

Turnaround Time

Samples will be tested on the same day or next routine working day following receipt.

Specimen

Female Endocervical swabs, vulvo-vaginal self -swabs or urine samples. Male urethral swabs or urine samples. Eye swabs are also processed.

Container

Genprobe Aptima Endocervical and urethral swab collection kit (use for female endocervical and male urethral samples, also for eye swabs). Genprobe Aptima vaginal swab collection kit (use for female vulvo-vaginal self -swabs). Genprobe Aptima urine collection kit (use for male and female urines). The swabs are available from the laboratory and can be stored for several months at room temperature. An expiry date is printed on the package. Please check EVERY swab before using.

Urine samples for detection of Chlamydia trachomatis must be collected into a urine collection cup or plain white capped universal bottle. The first 20-30mls of voided urine (not midstream) is required.

Collection

Specimen collection guides are available on request from the laboratory

Swab samples

Female Vulvo-vaginal swab 

Walls of the vagina must be touched when collecting sample to ensure that moisture is absorbed by the swab. Care must be taken when the swab is placed inside the transport tube that the fluid contained inside the transport container is not spilt or discarded. A new sample must be collected if this occurs.

Endocervical swabs

The large tipped white cleaning swab should be used to remove excess mucus from the cervical area then discarded. Only the smaller swab with the blue shaft should be used for taking the sample before being broken off into the transport tube at the score line on the shaft.

Care must be taken when the swab is placed inside the transport tube that the fluid contained inside the transport container is not spilt or discarded. A new sample must be collected if this occurs.

Male urethral swabs

Patient should not have urinated for at least one hour prior to collection.

Only the smaller swab with the blue shaft should be used for taking the sample before being broken off into the transport tube at the score line on the shaft.

Care must be taken when the swab is placed inside the transport tube that the fluid contained inside the transport container is not spilt or discarded. A new sample must be collected if this occurs.

Specimens in sample transport media can be stored at 2°C to 30°C and are stable for 60 days after collection.

Urine samples 

Patient should not have urinated for at least one hour prior to collection.
Female patients should not cleanse labial area prior to collecting urine sample.
Urine samples for detection of Chlamydia trachomatis must be collected into a urine collection cup or plain white capped universal bottle. The first 20-30mls of voided urine (not midstream) is required.

2ml of urine should be transferred into the urine specimen transport tube using the disposable pipette provided in the kit. The correct volume of urine has been added when the fluid level is between the two black fill lines on the urine specimen transport tube label. Urine MUST be pipetted into the urine specimen transport tube within 24 hours of collection or stored at 2°C to 30°C in the primary universal container but MUST reach the laboratory within 24 hours to allow transfer of urine into a urine specimen tube.
Urine samples once in the urine specimen transport tube can be stored at room temperature and are stable for 30 days after collection.

Causes for Rejection

Unlabelled or inadequately labelled samples. Urine samples in white topped universal container more than 24 hours old. Under filled or overfilled urine specimen transport tubes (sample must be added to between the two black fill lines on specimen transport tube). Swab samples where the foil cap has been pierced by the swab. Swab containers that have had the fluid discarded and contain only the swab.

Lab Handling

Samples should be processed as soon as possible