Detection of IgA autoantibodies against tissue
This is the primary Coeliac Disease screening test and all
requests for anti endomysial/gliadin antibodies will be screened
for the presence of anti tTG IgA. In addition all children under
the age of 16 will also have their serum IgA levels measured to
detect IgA deficiency
Please note: The anti tTG IgA assay should not be considered
diagnostic, but should be used in conjunction with all clinical and
Samples found to be tTG IgA positive or equivocal will be tested
for the presence of anti endomysial IgA. Samples found to be serum
IgA deficient, or where laboratory findings serum IgA deficiency,
will be presence of anti tTG IgG.
Request via ICE
Samples are tested daily Monday to Friday.
It is essential to give relevant clinical information
All tests are performed on the next routine working day after
reciept of the sample.
Red top tube.
Gold top (SST) tube will be accepted
Samples should be transported to the laboratory as soon as is
Causes for Rejection
Unlabelled/inadequately labelled sample
Inadequately completed request form
Delay in sample reaching laboratory
Aliquot and store at 4-8°C prior to testing and at -20°C or
below for up to 1 month after receipt.
Negative less than 7 U/ml
Equivocal 7-10 U/ml
Postive greater than 10 U/ml