Pathology Handbook

Cortisol (serum)

Synonyms

Compound F

Clinical Indications

In the investigation of possible Addison's disease (primary adrenocortical insufficiency) or Cushing's disease

Request Form 

Request on ICE

Availability

On request.

Specific Criteria

None

Patient Preparation

Cortisol shows considerable diurnal variation and samples must be collected between 9 and 10am for meaningful results to be obtained

Turnaround Time

Same day

Specimen

Serum in yellow top (SST) tube

Volume

2 mL

Container

Vacutainer gold top

Collection

Samples must be collected in the early morning

If an ACTH is also requested the samples must be collected at Southend Hospital and brought to the laboratory immediately

Causes for Rejection

Unlabelled or inadequately labelled sample

Reference Range

09:00: 170-540 nmol/L