Cryoglobulins, cryofibrinogen, cold agglutinins, immune
Cryoproteins are proteins which form a gel or precipitate on
cooling the serum or plasma and re-dissolve when warmed to
37'C. Cryoglobulins are immunoglobulins that exhibit this
phenomenon and may be either monoclonal (25%) or mixed (with (18%)
or without (50%) monoclonal components). The laboratory also
tests for cryofibrinogen
Cryoprotein studies are indicated in any patient showing
clinical manifestations including intolerance of cold with pain in
exposed area's, Raynaud's phenomenon, and skin manifestations
including purpura, urticaria and ulcers.
SST Serum and Plasma in Biochemistry EDTA tube
SAMPLE CONTAINERS MUST BE PRE-WARMED
Patients must be bled at Southend Hospital.
When patient is ready to be bled contact Pathology Reception to
obtain cryoprotein sampling tubes.
1. Take first blood sample into pre-warmed yellow top
(SST) tube and immediately place in sand bath at 37°C
2. Take second blood sample into pre-warmed lemon-topped
(EDTA) tube and immediately place in 37°C sand bath.
3. Take samples and request form (along with any other
samples taken at room temperature) to Pathology within 10 minutes
Causes for Rejection
Samples not collected using pre-warmed (37°C) collection tubes,
not transported in pre-warmed sand bath or delay in samples
Monoclonal cryoglobulins may be associated with immunocytomata
and are usually an IgM protein. Mixed cryoglobulins may be
associated with lymphoproliferative disorders, but the majority are