Pathology Handbook

Direct antiglobulin test (DAT)

Synonyms

DAT, Coombs test, DCT

Clinical Indications

Laboratory screening results suggest follow up test and/or Consultant direction examples; haemolytic anaemia, post transfusion

Request Form 

Request via ICE

Availability

On request.

Specific Criteria

On clinical indication only with guidance from Consultant Haematologist

Turnaround Time

Same day

Specimen

Whole blood in pink Blood transfusion (EDTA) tube or Purple (EDTA)

Samples must be labelled in full and signed by person collecting the blood, in accordance with the Blood Transfusion specimen acceptance policy

Volume

6 mL or 4.5 ml

Container

Pink ETDA BT

Collection

Samples should be transported to laboratory immediately

Causes for Rejection

Unlabelled /unsigned sample. Incorrect or inadequate patient identifiers provided. Insufficient sample volume. Poor sample quality due to deterioration, haemolysis, presence of clot