Samples found to be low level anti tTG IgA positive are assayed
for the presence of EMA IgA. Samples found to be IgA deficient are
assayed for the presence of EMA IgG.
Please note: The first line assay for the investigation of
Coeliac Disease and Dermatitis herpetiformis is anti tissue
Detection of IgA and IgG antibodies against the connective
tissue stroma covering individual smooth muscle fibres
Part of Profile
Request via ICE
The assay is performed as required.
It is essential to give relevant clinical information.
No special preparation is required.
Samples are tested in batches and the assay is generally
Red top tube
Gold top SST tube will be accepted.
Samples should be transported to the laboratory as soon as is
Aliquot and store at 4-8°C prior to testing and at -20°C or
below for up to 1 month after receipt.
Causes for Rejection
Unlabelled/inadequately labelled sample
Inadequately completed request form
Delay in sample reaching laboratory.
Sera are screened at a dilution of 1/5. In general titres
greater or equal to 1/5 are considered significant.