Pathology Handbook

Glomerular Basement Membrane Antibodies

Synonyms

GBM

Clinical Indications

Rapidly progressive glomerulonephritis (RPGN)

Goodpasture's Disease

Acute renal failure

Please note: Early diagnosis is important as untreated patients will suffer extensive tissue damage which in extreme cases may result in death. It has been noted that 10-35% of patients with anti GBM antibodies also have anti-neutrophil antibodies (ANCA).

Test Includes

Detection of IgG antibodies against the non-collagenous portion of type IV collagen.

Request Form 

Request via ICE

Availability

Assay is performed daily as required.

Specific Criteria

It is essential to provide relevant clinical information.

It is essential to inform the laboratory by telephone of any urgent requests.

Patient Preparation

No special preparation is required.

Turnaround Time

All tests are performed on the next routine working day after receipt of the sample.

Specimen

Serum in red top tube

Gold top (SST) tube will be accepted

Volume

7 ml

Container

Vacutainer red top

Collection

Samples should be transported to the laboratory as soon as is practicably possible.

Causes for Rejection

Unlabelled/inadequately labelled sample

Inadequately completed request form

Delay in sample reaching laboratory.

Laboratory Handling 

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Reference Range

Samples will be tested for the presence of anti GBM by Enzyme Immunoassay (EIA).
Negative <20 U/mL
Positive   >20 U/mL