Pathology Handbook

Islet Cell Antibody

Synonyms

ICA

Clinical Indications

Early Type I Diabetes. First degree relatives of patients with Type I Diabetes.

Please note: Islet Cell antibodies are found in approximately 70-80% of patients with Type I Diabetes, in approximately 50-70% of patients with latent autoimmune diabetes in adults (LADA), in approximately 5-10% of relatives of patients with Type I Diabetes and in autoimmune gestational diabetes. Antibodies are found in the early stages of the disease and gradually disappear over time.

Test Includes

Detection of IgG antibodies to a number of antigens in the cytoplasm and membrane of the islet of Langerhans cells.

Request Form 

Request via ICE.

Availability

The assay is performed weekly.

Specific Criteria

It is essential to provide relevant clinical information.

Patient Preparation

No special preparation is required.

Turnaround Time

All samples are generally sent to  referral laboratory within 3 working days of receipt in the laboratory. The samples are batched and the assay is performed once per week.

Specimen

Red top tube.

Gold top tube will be accepted. 

Volume

7 ml

Container

Vacutainer red top

Collection

Samples should be transported to the laboratory as soon as is practicably possible.

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample

Inadequately completed request form

Delay in sample reaching laboratory.

Reference Range

Samples are tested for the presence of anti Islet Cell antibodies by indirect immunofluorescence and the result reported as Positive or Negative.