Islet Cell Antibody
Early Type I Diabetes. First degree relatives of patients with
Type I Diabetes.
Please note: Islet Cell antibodies are found in
approximately 70-80% of patients with Type I Diabetes, in
approximately 50-70% of patients with latent autoimmune diabetes in
adults (LADA), in approximately 5-10% of relatives of patients with
Type I Diabetes and in autoimmune gestational diabetes. Antibodies
are found in the early stages of the disease and gradually
disappear over time.
Detection of IgG antibodies to a number of antigens in the
cytoplasm and membrane of the islet of Langerhans cells.
Request via ICE.
The assay is performed weekly.
It is essential to provide relevant clinical information.
No special preparation is required.
All samples are generally sent to referral
laboratory within 3 working days of receipt in the
laboratory. The samples are batched and the assay is performed once
Red top tube.
Gold top tube will be accepted.
Samples should be transported to the laboratory as soon as is
Aliquot and store at 4-8°C prior to testing and at -20°C or
below for up to 1 month after receipt.
Causes for Rejection
Unlabelled/inadequately labelled sample
Inadequately completed request form
Delay in sample reaching laboratory.
Samples are tested for the presence of anti Islet Cell
antibodies by indirect immunofluorescence and the result reported
as Positive or Negative.