Pathology Handbook

Leptospira microscopy


Weil's Disease

Test Includes

Microscopy for presence of Spirochaetes

Clinical Indications

Spirochaetes appear in the urine in the second phase of leptospirosis; this is 7 or more days post infection

Request Form 

Request via ICE


On request during routine working hours

Specific Criteria

Please give detailed clinical information including date of onset / contact with suspected infection
If culture for Leptospira is required blood cultures should also be collected

Turnaround Time

Same day


Urine, white topped universal container; do not collect into boric acid (red top) as the crystals may interfere with microscopy


5 ml


Urine bottle white top


Samples should be transported to laboratory immediately

Causes for Rejection

Unlabelled / inadequate sample
Use of wrong container (see above)
Delay in transport to laboratory

Lab Handling

Process on receipt