Pathology Handbook

M2 Mitochondrial Antibodies

Clinical Indications

Primary Biliary Cirrhosis (PBC)

Autoimmune Chronic Active Hepatitis

Please note: M2 antibodies are strongly associated with PBC in >95% of cases. The antibody is also found in chronic liver disease (30%) and Systemic Sclerosis (15%).

Test Includes

Detection of IgG antibodies against the E2 component of the pyruvate dehydrogenase complex.

Request Form 

Request via ICE.


Assay performed when sufficient samples received.

Specific Criteria

It is essential to provide relevant clinical information.

Patient Preparation

No special preparation is required.

Turnaround Time

All samples are generally sent to referral laboratory within 3 working days of receipt in the laboratory. The assay is performed when sufficient samples are received.


Red top tube. Gold top tube will be accepted.


7 ml


Vacutainer red top


Samples should be transported to the laboratory as soon as is practicably possible.

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample.

Inadequately completed request form.

Delay in sample reaching laboratory.

Reference Range

A qualitative result is given and samples are reported as Positive, Weak Positive or Negative.