Pathology Handbook

MI 2 Antibodies

Clinical Indications


(Specialist request only)

Please note: Mi-2 antibody is seen in 25% of adults with Dermatomyositis, in 10-15% of patients with Juvenile Dermatomyositis and in less than 1% of patients with polymyositis. MI-2 antibodies are generally of high titre, and show no variation during the course of the disease or its treatment.

Test Includes

Detection of IgG autoantibodies against the saline extracted cellular antigen Mi-2.

Request Form 

Request via ICE.


Assay is performed as required.

Specific Criteria

It is essential to provide relevant clinical information.

Patient Preparation

No special preparation is required.

Turnaround Time

All samples are generally sent to referral laboratory within 3 working days of receipt in the laboratory. The assay is performed as required.


Red top tube. Gold top tube will be accepted.


7 ml


Vacutainer red top


Samples should be transported to the laboratory as soon as is practicably possible.

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample.

Inadequately completed request form.

Delay in sample reaching laboratory.

Reference Range

A qualitative result is obtained and samples are reported as Positive or Negative.