Neuronal Nuclear Antibody – Anti Hu antibody
(specialist request only)
Subacute sensory neuropathy
Please note: Small cell lung carcinomas (SCLC) are found in
80% of cases. Other reported tumours include neuroblastomas,
prostate tumours, rhabdosarcomas, seminomas and adencarcinomas of
the gall bladder. If this antibody is found, a search for an
underlying tumour should be directed towards detection of SCLC
which may not become clinically apparent until many months after
the onset of neurological symptoms.
Detection if autoantibodies against neuronal nuclei of both the
central and peripheral nervous systems.
Request via ICE.
Assay performed weekly.
It is essential to provide relevant clinical information.
No special preparation is required.
All samples are generally sent to referral
laboratory within 3 working days of receipt in the
laboratory. The assay is performed as required.
Serum in red top tube
Gold top (SST) tube will be accepted
Samples should be transported to the laboratory as soon as is
Aliquot and store at 4-8°C prior to testing and at -20°C or
below for up to 1 month after receipt.
Causes for Rejection
Unlabelled/inadequately labelled sample
Inadequately completed request form
Delay in sample reaching laboratory.
Qualitative result: reported as Positive or Negative.
Quantitative result: reported as a titre Normal Range
All positive samples are confirmed.
Additional tests may be performed depending on patterns seen or
clinical details. These may include anti Amphiphysin, anti
CV2/CRMP5, anti PNMA2 (Ma2/Ta), anti Tr, anti Ri and anti Yo.
Additional assays may be Immunofluorescence and/or Immunoblot