Pathology Handbook

Neuronal Nuclear Antibody – Anti Hu antibody



Clinical Indications

(specialist request only)

Subacute sensory neuropathy


Please note: Small cell lung carcinomas (SCLC) are found in 80% of cases. Other reported tumours include neuroblastomas, prostate tumours, rhabdosarcomas, seminomas and adencarcinomas of the gall bladder. If this antibody is found, a search for an underlying tumour should be directed towards detection of SCLC which may not become clinically apparent until many months after the onset of neurological symptoms.

Test Includes

Detection if autoantibodies against neuronal nuclei of both the central and peripheral nervous systems.

Request Form 

Request via ICE.


Assay performed weekly.

Specific Criteria

It is essential to provide relevant clinical information.

Patient Preparation

No special preparation is required.

Turnaround Time

All samples are generally sent to referral laboratory within 3 working days of receipt in the laboratory. The assay is performed as required.


Serum in red top tube

Gold top (SST) tube will be accepted


7 ml


Vacutainer red top


Samples should be transported to the laboratory as soon as is practicably possible.

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample

Inadequately completed request form

Delay in sample reaching laboratory.

Reference Range

Qualitative result: reported as Positive or Negative.

Quantitative result: reported as a titre Normal Range <1/200

All positive samples are confirmed.

Additional tests may be performed depending on patterns seen or clinical details. These may include anti Amphiphysin, anti CV2/CRMP5, anti PNMA2 (Ma2/Ta), anti Tr, anti Ri and anti Yo. Additional assays may be Immunofluorescence and/or Immunoblot assays.