Pathology Handbook

Neuronal Nuclear Antibody – Anti Ri antibody


Anna 2

Clinical Indications

(specialist request only)

Axial ataxia



Please note: Breast or small cell liver carcinoma (SCLC) are found in 75% of cases, carcinomas of the ovaries, fallopian tubes, bladder and cervix are present less frequently. Detection of this antibody should prompt a search for an underlying tumour, primarily breast or SCLC which may be absent or occult.

Test Includes

Detection of autoantibodies against neuronal nuclei of the central nervous system only.

Request Form 

Request via ICE.


Assay performed as required.

Specific Criteria

It is essential to provide relevant clinical information.

Patient Preparation

No special preparation is required.

Turnaround Time

All samples are generally sent to  referral laboratory within 3 working days of receipt in the laboratory. The assay is performed as required.


Serum in red top tube

Gold top (SST) tube will be accepted


7 ml


Vacutainer red top


Samples should be transported to the laboratory as soon as is practicably possible.

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample

Inadequately completed request form

Delay in sample reaching laboratory.

Reference Range

Qualitative result: reported as Positive or Negative.

Quantitative result: reported as a titre Normal Range <1/200

All positive samples are confirmed.

Additional tests may be performed depending on patterns seen or clinical details. These may include anti Amphiphysin, anti CV2/CRMP5, anti PNMA2 (Ma2/Ta), anti Tr, anti Hu, and anti Yo. Additional assays may be Immunofluorescence and/or Immunoblot assays.