Non Jo-1 Synthetase Antibodies
(specialist request only)
Please note: Jo-1 antibodies are found in 20-40% of
patients with myositis. The non Jo-1 Synthetase antibodies are
found in 5-10% of adult patients with myositis, PL-7 being seen in
approximately 5% and PL-12 in 3%. The other antibodies are less
common. The primary assay for Polymyositis or Dermatomyositis is
the ENA screen which includes Jo-1 (histidyl tRNA synthetase).
Detection of autoantibodies to a family of aminoacyl-tRNA
synthetases including PL-7 (threonyl tRNA synthetase); PL-12
(alanyl tRNA synthetase); EJ (glycyl tRNA synthetase) and OJ
(isoleucyl tRNA synthetase).
Request via ICE.
Assay performed as required.
It is essential to provide relevant clinical information.
No special preparation is required.
All samples are generally sent to referral
laboratory within 3 working days of receipt in the
laboratory. The assay is performed as required.
Serum in red top tube
Gold top (SST) tube will be accepted
Samples should be transported to the laboratory as soon as is
Aliquot and store at 4-8°C prior to testing and at -20°C or
below for up to 1 month after receipt.
Causes for Rejection
Unlabelled/inadequately labelled sample
Inadequately completed request form
Delay in sample reaching laboratory.
A qualitative result is given and samples are reported as
Positive, Weak Positive or Negative.