Pathology Handbook

PM-Scl Antibodies

Clinical Indications

Polymyositis/scleroderma overlap syndrome

(Specialist request only)

Please note: This autoantibody may also be found in patients with dermatomyositis/polymyositis or systemic sclerosis occurring alone.

Test includes

Detection of IgG antibodies to a complex of 11 cell proteins.

Request Form 

Request on ICE


Assay is performed as required.

Patient preparation

No special preparation is required.

Specific Criteria

It is essential to provide relevant clinical information.

Turnaround Time

All samples are generally sent to referral laboratory within 3 working days of receipt in the laboratory. The assay is performed as required.


Red top tube. Gold top tube will be accepted.


7 ml


Vacutainer red top 


Samples should be transported to the laboratory as soon as is practicably possible.

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample.

Inadequately completed request form.

Delay in sample reaching laboratory.

Reference Range

A qualitative result is given and samples are reported as Positive or Negative.