Pathology Handbook

Purkinje Cell Antibodies – Anti Tr



Clinical Indications

Cerebellar ataxia

(Specialist request only)

Please note: There is a strong link between the presence of this antibody and paraneoplastic cerebellar degeneration in patients with Hodgkin's disease (80%).

Test includes

Detection of autoantibodies to antigens expressed in Purkinje neurones.

Request Form 

Request on ICE


Assay is performed weekly.

Patient preparation

No special preparation is required.

Specific Criteria

It is essential to provide relevant clinical information.

Turnaround Time

All samples are generally sent to referral laboratory   within 3 working days of receipt in the laboratory. Samples are batched and the assay is performed once per week.


Red top tube. Gold top tube will be accepted.


7 ml


Vacutainer red top


Samples should be transported to the laboratory as soon as is practicably possible.

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample.

Inadequately completed request form.

Delay in sample reaching laboratory.

Reference Range

Results are reported as a titre.

Additional tests may be performed depending on patterns seen or clinical details. These may include anti Amphiphysin, anti CV2/CRMP5, anti PNMA2 (Ma2/Ta), anti Ri, anti Hu, and anti Yo. Additional assays may be Immunofluorescence and/or Immunoblot assays.