Pathology Handbook

Purkinje Cell Antibodies – Anti Yo




Clinical Indications

Cerebellar degeneration

(Specialist request only)

Please note: Carcinoma of the ovary or breast are found in 90% of cases. Other reported tumours include those of the uterus, fallopian tube and lung. Apart from rare exceptions, anti Yo antibodies occur with gynaecological cancers and their presence warrants the search for an occult tumour.

Test includes

Detection of autoantibodies against organelles in the cytoplasm of Purkinje cells.

Request Form 

Request on ICE


Assay is performed weekly.

Patient preparation

No special preparation is required.

Specific Criteria

It is essential to provide relevant clinical information.

Turnaround Time

All samples are generally sent to referral laboratory within 3 working days of receipt in the laboratory. Samples are batched and the assay is performed once per week.


Red top tube. Gold top tube will be accepted.


7 ml


Vacutainer red top


Samples should be transported to the laboratory as soon as is practicably possible.

Lab Handling

Aliquot and store at 4-8°C prior to testing and at -20°C or below for up to 1 month after receipt.

Causes for Rejection

Unlabelled/inadequately labelled sample.

Inadequately completed request form.

Delay in sample reaching laboratory.

Reference Range

Qualitative result: reported as Positive or Negative.

Quantitative result: reported as a titre Normal Range <1/200 All positive samples are confirmed. Additional tests may be performed depending on patterns seen or clinical details. These may include anti Amphiphysin, anti CV2/CRMP5, anti PNMA2 (Ma2/Ta), anti Ri, anti Hu, and anti Tr. Additional assays may be Immunofluorescence and/or Immunoblot assays.